Journal of materials science. Materials in medicine, 2019-11, Vol.30 (11), p.123-17, Article 123
2019
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- Autor(en) / Beteiligte
- Titel
- Diffusion tensor imaging predicting neurological repair of spinal cord injury with transplanting collagen/chitosan scaffold binding bFGF
- Ist Teil von
- Journal of materials science. Materials in medicine, 2019-11, Vol.30 (11), p.123-17, Article 123
- Ort / Verlag
- New York: Springer US
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Prognosis and treatment evaluation of spinal cord injury (SCI) are still in the long-term research stage. Prognostic factors for SCI treatment need effective biomarker to assess therapeutic effect. Quantitative diffusion tensor imaging (DTI) may become a potential indicators for assessing SCI repair. However, its correlation with the results of locomotor function recovery and tissue repair has not been carefully studied. The aim of this study was to use quantitative DTI to predict neurological repair of SCI with transplanting collagen/chitosan scaffold binding basic fibroblast growth factor (bFGF). To achieve our research goals, T10 complete transection SCI model was established. Then collagen/chitosan mixture adsorbed with bFGF (CCS/bFGF) were implanted into rats with SCI. At 8 weeks after modeling, implanting CCS/bFGF demonstrated more significant improvements in locomotor function according to Basso-Beattie-Bresnahan (BBB) score, inclined-grid climbing test, and electrophysiological examinations. DTI was carried out to evaluate the repair of axons by diffusion tensor tractgraphy (DTT), fractional anisotropy (FA) and apparent diffusion coefficient (ADC), a numerical measure of relative white matter from the rostral to the caudal. Parallel to locomotor function recovery, the CCS/bFGF group could significantly promote the regeneration of nerve fibers tracts according to DTT, magnetic resonance imaging (MRI), Bielschowsky’s silver staining and immunofluorescence staining. Positive correlations between imaging and locomotor function or histology were found at all locations from the rostral to the caudal ( P < 0.0001). These results demonstrated that DTI might be used as an effective predictor for evaluating neurological repair after SCI in experimental trails and clinical cases.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0957-4530eISSN: 1573-4838DOI: 10.1007/s10856-019-6322-yTitel-ID: cdi_proquest_miscellaneous_2312273360
- Format
- –
- Schlagworte
- Animals, Anisotropy, Axons, Binding, Biomarkers, Biomaterials, Biomedical Engineering and Bioengineering, Biomedical materials, Blood Vessel Prosthesis, Ceramics, Chemistry and Materials Science, Chitosan, Chitosan - chemistry, Chitosan - therapeutic use, Collagen, Collagen - chemistry, Collagen - therapeutic use, Composites, Diffusion coefficient, Diffusion effects, Diffusion Tensor Imaging, Dithiothreitol, Evaluation, Fibroblast growth factor 2, Fibroblast Growth Factor 2 - chemistry, Fibroblast Growth Factor 2 - pharmacology, Glass, Growth factors, Histology, Immunofluorescence, Magnetic resonance imaging, Materials Science, Mathematical analysis, Medical prognosis, Natural Materials, NMR, Nuclear magnetic resonance, Polymer Sciences, Rats, Recovery, Regeneration, Regenerative Medicine/Tissue Engineering, Repair, Scaffolds, Spinal cord injuries, Spinal Cord Injuries - therapy, Staining, Substantia alba, Surfaces and Interfaces, Tensors, Thin Films, Tissue Engineering Constructs and Cell Substrates