Details

Autor(en) / Beteiligte

• Mei, You‐Min
• Li, Lu
• Wang, Xiao‐Qian
• Zhang, Min
• Zhu, Li‐Fang
• Fu, Yong‐Wei
• Xu, Yan

Titel

AGEs induces apoptosis and autophagy via reactive oxygen species in human periodontal ligament cells

Ist Teil von

• Journal of cellular biochemistry, 2020-08, Vol.121 (8-9), p.3764-3779

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2020

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• The apoptosis of human periodontal ligament cells (HPDLCs) may be an important factor of the negative effect of advanced glycation end products (AGEs) on the periodontal tissue of diabetic patients. However, the pathways or potential effects of apoptosis in AGEs‐treated HPDLCs have not been fully elucidated. Autophagy is closely related to apoptosis. Herein, we investigated the potential mechanism of apoptosis and autophagy in HPDLCs treated with AGEs via an in vitro model. We found that AGEs‐treated HPDLCs showed a time‐ and concentration‐dependent reduction in the cell survival rate. The mitochondrial‐dependent apoptosis was induced in AGEs‐treated HPDLCs, as confirmed by the mitochondrial membrane potential depolarization, decreased Bcl‐2 expression, increased Bax expression, and increased caspase‐3 and PARP cleavage. Autophagy was also induced in AGEs‐treated HPDLCs, as indicated by the conversion of LC3‐II/LC3‐I and the presence of autophagosomes. Interestingly, our study results suggested that apoptosis and autophagy were related to reactive oxygen species (ROS) production. In addition, AGEs‐induced autophagy acted as a latent factor in decreasing the generation of ROS in HPDLCs and protecting against the AGEs‐induced apoptosis. In summary, our study shows that ROS are essential in AGEs‐induced HPDLCs apoptosis and autophagy, which may be a molecular mechanism for the repairment of ROS‐induced damage in HPDLCs treated with AGEs to promote cell survival. The present study might provide new insights into the therapeutic targeting of HPDLCs autophagy, which could be an additional strategy for periodontitis in patients with diabetes mellitus. Advanced glycation end products induce an autophagic response through the ERK signaling pathway mediated by reactive oxygen species, thereby inhibiting apoptosis.