The New phytologist, 2020-02, Vol.225 (4), p.1618-1634
2020
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Genome-wide analysis of coding and non-coding RNA reveals a conserved miR164-NAC regulatory pathway for fruit ripening
- Ist Teil von
- The New phytologist, 2020-02, Vol.225 (4), p.1618-1634
- Ort / Verlag
- England: Wiley
- Erscheinungsjahr
- 2020
- Quelle
- Wiley Online Library - AutoHoldings Journals
- Beschreibungen/Notizen
- Kiwifruit (Actinidia spp.) is a climacteric fruit with high sensitivity to ethylene, influenced by multiple ethylene-responsive structural genes and transcription factors. However, the roles of other post-transcriptional regulators (e.g. miRNAs) necessary for ripening remain elusive. High-throughput sequencing sRNAome, degradome and transcriptome methods were used to identify further contributors to ripening control in the kiwifruit (A. deliciosa cv ‘Hayward’). Two NAM/ATAF/CUC domain transcription factors (AdNAC6 and AdNAC7), both predicted targets for miR164, showed significant upregulation by exogenous ethylene. Gene expression analysis and luciferase reporter assays indicated that Ade-miR164 and one of its precursor miRNAs (Ade-MIR164b) were repressed by ethylene treatment and negatively correlated with AdNAC6/7 expression. Subsequent analysis indicated that both AdNAC6 and AdNAC7 proteins are transcriptional activators and physically bind the promoters of AdACS1 (1-aminocyclopropane-1-carboxylate synthase), AdACO1 (1-aminocyclopropane-1-carboxylic acid oxidase), AdMAN1 (endo-β-mannanase) and AaTPS1 (terpene synthase). Moreover, subcellular analysis indicated that the location of the AdNAC6/7 proteins was influenced by Ade-miR164. Multiple omics-based approaches revealed a novel regulatory link for fruit ripening that involved ethylene-miR164-NAC. The regulatory pathway for miR164-NAC is present in various fruit (e.g. Rosaceae fruit, citrus, grape), with implications for fruit ripening regulation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0028-646XeISSN: 1469-8137DOI: 10.1111/nph.16233Titel-ID: cdi_proquest_miscellaneous_2300187763
- Format
- –
- Schlagworte
- Actinidia, Actinidia - physiology, Analysis, aroma, Carboxylic acids, cell wall, Citrus fruits, degradome, DNA, Plant - genetics, Ethene, Ethylene, Fruit - growth & development, Fruit - metabolism, fruit ripening, Fruits, Gene expression, Genome, Plant, Genome-Wide Association Study, Genomes, Identification methods, Kiwifruit, Mannanases, MicroRNAs, miR164, NAC, Non-coding RNA, Nucleic acids, Phylogeny, Promoter Regions, Genetic, Proteins, Ribonucleic acid, Ripening, RNA, RNA, Plant - genetics, RNA, Plant - metabolism, RNA, Untranslated - genetics, RNA, Untranslated - metabolism, Terpene synthase, Transcription, Transcription factors, transcriptome