Details

Autor(en) / Beteiligte

• Zhang, Jia-Qing
• Gao, Bin-Wen
• Guo, Hong-Xia
• Ren, Qiao-Ling
• Wang, Xian-Wei
• Chen, Jun-Feng
• Wang, Jing
• Zhang, Zi-Jing
• Ma, Qiang
• Xing, Bao-Song

Titel

miR-181a promotes porcine granulosa cell apoptosis by targeting TGFBR1 via the activin signaling pathway

Ist Teil von

• Molecular and cellular endocrinology, 2020-01, Vol.499, p.110603-110603, Article 110603

Ort / Verlag

Ireland: Elsevier B.V

Erscheinungsjahr

2020

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Activin/Smad3 signaling plays a pivotal role in follicle development and atresia. However, the precise mechanisms underlying this process are not yet fully understood. Herein, we identified miR-181a as a central component of activin/Smad3-mediated follicle atresia. miR-181a was strikingly upregulated in porcine atretic follicles, which induced the apoptosis of porcine granulosa cells (GCs) in vitro. Furthermore, the transforming growth factor-β type 1 receptor (TGFBR1) was confirmed as a direct target of miR-181a by bioinformatics analysis and luciferase assays. Transfection with an miR-181a agomir repressed the TGFBR1 mRNA and protein levels. In addition, TGFBR1 overexpression repressed GC apoptosis, whereas TGFBR1 inhibition promoted GC apoptosis. miR-181a overexpression downregulated the phosphorylation of Smad3 and blocked the activation of TGF-β signaling. Moreover, activin A downregulated miR-181a expression and upregulated the TGFBR1 and p-Smad3 protein levels. Collectively, these data suggest that miR-181a regulates porcine GC apoptosis by targeting TGFBR1 via the activin signaling pathway. •miR-181a promotes porcine granulsoa cell apoptosis.•mir-181a affects the production of subcellular location of p-smad3.•TGFBR1 is a direct target of miR-181a.•Inhibition of TGFBR1 triggers porcine granulsoa cell apoptosis.•miR-181a regulates porcine GC apoptosis by targeting TGFBR1 via the activin signaling pathway.