Details

Autor(en) / Beteiligte

• Xie, Yingying
• Zhang, Kang
• Zhang, Kai
• Zhang, Jingyan
• Wang, Lei
• Wang, Xurong
• Hu, Xuequan
• Liang, Zijing
• Li, Jianxi

Titel

Toll‐like receptors and high mobility group box 1 in granulosa cells during bovine follicle maturation

Ist Teil von

• Journal of cellular physiology, 2020-04, Vol.235 (4), p.3447-3462

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Toll‐like receptors (TLRs) are present in the ovaries and reproductive tract of various mammals. The biological function of TLR during ovulation is one of the main contents in the research of reproductive immunology. In this study, we found that messenger RNA levels of TLR1–TLR10 in granulosa cells were different, and TLRs and high mobility group box 1 (HMGB1) in granulosa cells of large follicles were significantly higher than those of small and middle follicles. Coimmunoprecipitation results showed that HMGB1 interacts with TLR2 in granulosa cells, especially large follicles. The result of immunohistochemistry showed that TLRs and HMGB1 were present in granulosa cell layer of ovarian follicles. We also found 25 mIU/ml follicle‐stimulating hormone (FSH) significantly upregulated the expression of TLRs and HMGB1. These results suggest that TLR2/4 and HMGB1 in granulosa cells may be involved in the ovarian innate immune and ovarian follicular maturation, regulated by FSH. However, further research of the function and mechanisms of TLRs and HMGB1 in granulosa cells are needed. In granulosa cells of bovine ovarian follicles, the expression of TLR2/4 shows a tendency to increase with follicular growth. The suitable concentration of FSH stimulates granulosa cells to release HMGB1, which interact with TLR2/4 and activate the downstream signaling pathway to secrete cytokines involved in innate immunity, follicular dominance, and ovulation. We speculate that the underlying mechanism is related to PKC and Ca2+.