Details

Autor(en) / Beteiligte

• Vail, Daniel J.
• Somoza, Rodrigo A.
• Caplan, Arnold I.
• Khalil, Ahmad M.

Titel

Transcriptome dynamics of long noncoding RNAs and transcription factors demarcate human neonatal, adult, and human mesenchymal stem cell‐derived engineered cartilage

Ist Teil von

• Journal of tissue engineering and regenerative medicine, 2020-01, Vol.14 (1), p.29-44

Ort / Verlag

England: Hindawi Limited

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The engineering of a native‐like articular cartilage (AC) is a long‐standing objective that could serve the clinical needs of millions of patients suffering from osteoarthritis and cartilage injury. An incomplete understanding of the developmental stages of AC has contributed to limited success in this endeavor. Using next generation RNA sequencing, we have transcriptionally characterized two critical stages of AC development in humans—that is, immature neonatal and mature adult, as well as tissue‐engineered cartilage derived from culture expanded human mesenchymal stem cells. We identified key transcription factors (TFs) and long noncoding RNAs (lncRNAs) as candidate drivers of the distinct phenotypes of these tissues. AGTR2, SCGB3A1, TFCP2L1, RORC, and TBX4 stand out as key TFs, whose expression may be capable of reprogramming engineered cartilage into a more expandable and neonatal‐like cartilage primed for maturation into biomechanically competent cartilage. We also identified that the transcriptional profiles of many annotated but poorly studied lncRNAs were dramatically different between these cartilages, indicating that lncRNAs may also be playing significant roles in cartilage biology. Key neonatal‐specific lncRNAs identified include AC092818.1, AC099560.1, and KC877982. Collectively, our results suggest that tissue‐engineered cartilage can be optimized for future clinical applications by the specific expression of TFs and lncRNAs.