Chemical communications (Cambridge, England), 2019-09, Vol.55 (78), p.11671-11674
2019
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors
- Ist Teil von
- Chemical communications (Cambridge, England), 2019-09, Vol.55 (78), p.11671-11674
- Ort / Verlag
- England: Royal Society of Chemistry
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery. Fluorogenic DNA fork substrates have been designed for use in high-throughput discovery of potential anti-cancer drugs which inhibit DNA repair.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1359-7345eISSN: 1364-548XDOI: 10.1039/c9cc05476fTitel-ID: cdi_proquest_miscellaneous_2287516078
- Format
- –
- Schlagworte
- Design optimization, Dimerization, DNA Repair, DNA-Binding Proteins - chemistry, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, Endonucleases - chemistry, Endonucleases - genetics, Endonucleases - metabolism, Fluorescence, Humans, Life Sciences, Nuclease, Oligonucleotides - chemistry, Oligonucleotides - metabolism, Repair, Substrate Specificity, Substrates, Temperature