Details

Autor(en) / Beteiligte

• Díaz-Fernández, David
• Aguiar, Tatiana Q.
• Martín, Victoria Isabel
• Romaní, Aloia
• Silva, Rui
• Domingues, Lucília
• Revuelta, José Luis
• Jiménez, Alberto

Titel

Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains

Ist Teil von

• Bioresource technology, 2019-12, Vol.293, p.122054-122054, Article 122054

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2019

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• [Display omitted] •Microbial lipids from renewable and waste materials.•Alleviating regulatory bottlenecks enhanced the production of microbial lipids.•Ashbya gossypii can use industrial wastes for the production of microbial lipids.•About 40% of CDW in lipid content was obtained using industrial wastes as feedstocks. This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main Δ9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks.