Details

Autor(en) / Beteiligte

• Bergantini, L
• Cameli, P
• d’Alessandro, M
• Vagaggini, C
• Refini, RM
• Landi, C
• Pieroni, MG
• Spalletti, M
• Sestini, P
• Bargagli, E

Titel

NK and NKT-like cells in granulomatous and fibrotic lung diseases

Ist Teil von

• Clinical and experimental medicine, 2019-11, Vol.19 (4), p.487-494

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2019

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Abtract Background The pathogenetic and regulatory roles of natural killer (NK) and natural killer T-like cells in interstitial lung diseases (ILDs), fibrotic and granulomatous of unknown etiology are unclear. Objectives Here we investigated NK and NKT-like cells in peripheral blood (PB) and Bronchoalveolar lavage (BAL) from patients with ILDs. Method 190 patients (94 male mean age 61 ± 14.3 years) and 8 controls undergoing bronchoscopy for ILD diagnostic work-up were enrolled consecutively; 115 patients sarcoidosis, 24 chronic fibrotic hypersensitivity pneumonitis and 43 patients other ILDs [32 idiopathic pulmonary fibrosis (IPF) and 11 non-specific interstitial pneumonia (NSIP)]. PB and BAL were processed by flow cytometry using monoclonal antibodies to differentiate NK and NKT-like cells. Results NK% in BAL was significantly different among ILDs ( p  = 0.02). Lower NK% was observed in BAL from sarcoidosis than other ILDs ( p  < 0.05). Similar findings were observed for NKT-like, whereas no differences were found for PB NK%. Difference of NK% was observed between BAL and PB in all groups ( p  < 0.001). Sarcoidosis patients reported the best area under the curve for NKT-like (AUC = 0.678, p  = 0.0015) and NK cells (AUC = 0.61, p  = 0.001). In the IPF-NSIP subgroup, NK% cell was inversely correlated with FVC% ( r  = − 0.34, p  = 0.03) and DLCO% ( r  = − 0.47, p  = 0.0044). Conclusions NK and NKT-like were expressed differently in BAL from patients with different ILD and were significantly depleted in sarcoidosis respect to other ILDs. This suggests that these cells may play a protective role in the pathogenesis of sarcoidosis.