Details

Autor(en) / Beteiligte

• Wei, Tao
• Ren, Pingping
• Huang, Linli
• Ouyang, Zicheng
• Wang, Ziying
• Kong, Xiangfeng
• Li, Tiejun
• Yin, Yulong
• Wu, Yongning
• He, Qinghua

Titel

Simultaneous detection of aflatoxin B1, ochratoxin A, zearalenone and deoxynivalenol in corn and wheat using surface plasmon resonance

Ist Teil von

• Food chemistry, 2019-12, Vol.300, p.125176-125176, Article 125176

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2019

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• •A SPR method for simultaneous analysis of AFB1, OTA, ZEN and DON in corn and wheat is presented.•A specific and sensitive SPR sensor chip was fabricated based on self-assembled monolayer.•Mycotoxins were detected with indirect competitive immunoassay format.•Application of a SPR technique significantly increases the sensitivity of the method. Mycotoxins are toxic metabolites produced by fungi or molds, which may cause serious harm to human health through polluted cereal foods. In order to measure the typical mycotoxin contaminations in wheat and corn, a surface plasmon resonance (SPR) method was established using SPR sensor chip that was fabricated based on self-assembled monolayer. The minimum detection limit of aflatoxin B1, ochratoxin A, zearalenone and deoxynivalenol were identified as 0.59 ng/mL, 1.27 ng/mL, 7.07 ng/mL and 3.26 ng/mL, respectively. The cross-reactivity for all four mycotoxins were demonstrated to be low. Moreover, the test data were compared with HPLC-MS/MS confirmatory analysis results and good agreement was found between them. In conclusion, the SPR method for simultaneously detecting four mycotoxins has been developed with high sensitivity, good linearity and specificity, which can meet the detection requirements of cereal foods.