Biochemistry (Moscow), 2019-06, Vol.84 (6), p.637-643
2019
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- Autor(en) / Beteiligte
- Titel
- Protective Effect of Peroxiredoxin 6 Against Toxic Effects of Glucose and Cytokines in Pancreatic RIN-m5F β-Cells
- Ist Teil von
- Biochemistry (Moscow), 2019-06, Vol.84 (6), p.637-643
- Ort / Verlag
- Moscow: Pleiades Publishing
- Erscheinungsjahr
- 2019
- Quelle
- SpringerLink
- Beschreibungen/Notizen
- Taking into account a special role of pancreatic β-cells in the development of diabetes mellitus, the effects of peroxiredoxin 6 (Prx6) on the viability and functional activity of rat insulinoma RIN-m5F β-cells were studied under diabetes-simulating conditions. For this purpose, the cells were cultured at elevated glucose concentrations or in the presence of proinflammatory cytokines (TNF-α and IL-1) known for their special role in the cytotoxic autoimmune response in diabetes. It was found that the increased glucose concentration of 23-43 mM caused death of 20-60% β-cells. Prx6 added to cells significantly reduced the level of reactive oxygen species and protected the RIN-m5F ß-cells from hyperglycemia, reducing the death of these cells by several fold. A measurement of insulin secretion by the RIN-m5F ß-cells showed a significant stimulatory effect of Prx6 on the insulin-producing activity of pancreatic β-cells. It should be noted that the stimulatory activity of Prx6 was detected during culturing the cells under both normal and unfavorable conditions. The regulation of the NF-ϰB signaling cascade could be one of the mechanisms of Prx6 action on β-cells, in particular, through activation of RelA/p65 phosphorylation at Ser536.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-2979eISSN: 1608-3040DOI: 10.1134/S0006297919060063Titel-ID: cdi_proquest_miscellaneous_2261262161
- Format
- –
- Schlagworte
- Animals, Beta cells, Biochemistry, Biomedical and Life Sciences, Biomedicine, Bioorganic Chemistry, Cell Death - physiology, Cell Line, Tumor, Cell Survival - physiology, Cytokines, Cytokines - metabolism, Cytokines - toxicity, Cytotoxicity, Diabetes, Diabetes mellitus, Glucose, Glucose - metabolism, Glucose - toxicity, Hyperglycemia, Inflammation, Inflammation Mediators - metabolism, Insulin, Insulin - biosynthesis, Insulin - metabolism, Insulin secretion, Insulin-Secreting Cells - drug effects, Insulin-Secreting Cells - metabolism, Insulinoma, Interleukin 1, Interleukin-1 - metabolism, Life Sciences, Microbiology, NF-kappa B - metabolism, Pancreas, Peroxiredoxin, Peroxiredoxin VI - physiology, Phosphorylation, Protected species, Rats, Reactive oxygen species, Reactive Oxygen Species - metabolism, Signal Transduction, Toxicity, Tumor Necrosis Factor-alpha - metabolism, Tumor necrosis factor-α, Viability