Details

Autor(en) / Beteiligte

• Huang, Mu-Yang
• Jiang, Xiao-Ming
• Xu, Yu-Lian
• Yuan, Luo-Wei
• Chen, Yu-Chi
• Cui, Guozhen
• Huang, Run-Yue
• Liu, Bo
• Wang, Yitao
• Chen, Xiuping
• Lu, Jin-Jian

Titel

Platycodin D triggers the extracellular release of programed death Ligand-1 in lung cancer cells

Ist Teil von

• Food and chemical toxicology, 2019-09, Vol.131, p.110537-110537, Article 110537

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Programmed death ligand-1 (PD-L1) is an important immune checkpoint for cancer immunotherapy in clinic. In this study, we reported that platycodin D, a natural product isolated from an edible and medicinal plant Platycodon grandiflorus (Jacq.) A. DC., down-regulated the protein level of PD-L1 in lung cancer cells. Flow cytometry and immunofluorescence assay showed a weaker surface PD-L1 signal in NCI–H1975 cells after the incubation with platycodin D (10 μM) for 15 min compared to the control group. Jurkat T cells showed enhancive interleukin-2 secretion when co-cultured with platycodin D-treated NCI–H1975 cells, suggesting that platycodin D-induced PD-L1 reduction increases the activation of Jurkat T cells. An augmentation of PD-L1 protein was detected in the cell culture medium from platycodin D treatment group. Chlorpromazine (60 μM) almost abolished the platycodin D-mediated PD-L1 extracellular release and restored the membrane PD-L1. Finally, hemolysis assay exhibited that platycodin D-triggered PD-L1 extracellular release was independent of the hemolytic mechanism. Taken together, our study demonstrates that platycodin D reduces the protein level of PD-L1 in lung cancer cells via triggering its release into the cell culture medium, which sheds new light for the application of natural products in cancer immunotherapy. •Platycodin D-triggered extracellular release of PD-L1 in lung cancer cells.•Jurkat T cells co-cultured with platycodin D-treated NCI–H1975 cells shows enhanced interleukin-2 secretion.•Chlorpromazine abolishes platycodin D-triggered extracellular release of PD-L1.