Details

Autor(en) / Beteiligte

• Fernández-Cuenca, Felipe
• Pérez-Palacios, Patricia
• Galán-Sánchez, Fátima
• López-Cerero, Lorena
• López-Hernández, Inmaculada
• López Rojas, Rafael
• Arca-Suárez, Jorge
• Díaz-de Alba, Paula
• Rodríguez Iglesias, Manuel
• Pascual, Alvaro

Titel

First identification of blaNDM-1 carbapenemase in blaOXA-94-producing Acinetobacter baumannii ST85 in Spain

Ist Teil von

• Enfermedades infecciosas y microbiologia clinica (English ed.), 2020-01, Vol.38 (1), p.11-15

Ort / Verlag

Elsevier España, S.L.U

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• AbstractIntroductionNDM-1 carbapenemase is spreading rapidly all over the world, but this metallo-beta-lactamase has just been detected for the first time in an Acinetobacter baumannii (Ab) isolate of the ST85 clone in Spain. The aim of this study was to characterize a NDM-1-producing carbapenem-resistant A. baumannii (CR-Ab) isolate submitted to the Andalusian PIRASOA [infection prevention program] referral laboratory. MethodsCarbapenemases were detected by PCR and Sanger DNA sequencing. Whole genome sequencing was performed by NGS (Miseq, Illumina). Resistance genes were identified with RESfinder, while MLSTfinder was used for sequence typing (ST). The genetic location of blaNDM-1 was determined by nuclease S-1/PFGE/hybridization with specific probe. ResultsThe isolate was susceptible to amikacin and tigecycline and belonged to the ST85 clone. blaOXA-94 and blaNDM-1 were identified by PCR and Sanger DNA sequencing, respectively. The resistance genes aadB, blaADC-25, blaNDM-1, blaOXA-94, msr(E), mph(E) and floR,sul2 were identified by NGS. The chromosome of the isolate contained a defective Tn125 transposon with blaNDM-1 flanked by the insertion sequences IS AbA125 and IS Aba14. The blaNDM-1 gene was only detected in the chromosomal DNA. ConclusionThis is the first time that blaNDM-1 has been detected and characterized in a blaOXA-94-producing CR-Ab isolate belonging to the ST85 clone in Spain.