Details

Autor(en) / Beteiligte

• Xu, Dongdong
• Yoshino, Tasuku
• Konishi, Junpei
• Yoshikawa, Hiroyuki
• Ino, Yasuko
• Yazawa, Ryosuke
• Nassif Lacerda, Samyra Maria dos Santos
• de França, Luiz Renato
• Takeuchi, Yutaka

Titel

Germ cell-less hybrid fish: ideal recipient for spermatogonial transplantation for the rapid production of donor-derived sperm

Ist Teil von

• Biology of reproduction, 2019-08, Vol.101 (2), p.492-500

Ort / Verlag

United States: Society for the Study of Reproduction

Erscheinungsjahr

2019

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• An interspecific hybrid marine fish that developed a testis-like gonad without any germ cells, i.e., a germ cell-less gonad, was produced by hybridizing a female blue drum Nibea mitsukurii with a male white croaker Pennahia argentata. In this study, we evaluated the suitability of the germ cell-less fish as a recipient by transplanting donor testicular cells directly into the gonads through the urogenital papilla. The donor testicular cells were collected from hemizygous transgenic, green fluorescent protein (gfp) (+/–) blue drum, and transplanted into the germ cell-less gonads of the 6-month-old adult hybrid croakers. Fluorescent and histological observations showed the colonization, proliferation, and differentiation of transplanted spermatogonial cells in the gonads of hybrid croakers. The earliest production of spermatozoa in a hybrid recipient was observed at 7 weeks post-transplantation (pt), and 10% of the transplanted recipients produced donor-derived gfppositive spermatozoa by 25 weeks pt. Sperm from the hybrid recipients were used to fertilize eggs from wild-type blue drums, and approximately 50% of the resulting offspring were gfppositive, suggesting that all offspring originated from donor-derived sperm that were produced in the transplanted gfp (+/–) germ cells. To the best of our knowledge, this is the first report of successful spermatogonial transplantation using a germ cell-less adult fish as a recipient. This transplantation system has considerable advantages, such as the use of comparatively simple equipment and procedures, and rapid generation of donor-derived spermatogenesis and offspring, and presents numerous applications in commercial aquaculture. Summary Sentence Hybrid marine sciaenid fish showing germ cell-less phenotype are ideal recipients for developing spermatogonial transplantation in adult fish, and enable the rapid production of donor-derived sperm in 7 weeks post-transplantation.