Details

Autor(en) / Beteiligte

• Wang, Jin‐Ya
• Zhang, Dao‐Qi
• Cao, Qian
• Qiao, Xiao‐Qin
• Zhou, Guo‐Ping

Titel

miR‐939‐5p decreases the enrichment of RNA polymerase II in the promoter region of CD2AP involved in nephrotic syndrome

Ist Teil von

• Journal of cellular biochemistry, 2019-07, Vol.120 (7), p.11366-11374

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2019

Quelle

Wiley Online Library

Beschreibungen/Notizen

• The expression changes of CD2‐associated protein (CD2AP) can lead to kidney diseases with proteinuria, including nephrotic syndrome (NS). A recent study reported that miRNAs may be important transcriptional regulators. In this study, we found increased expression of miR‐939‐5p and decreased expression of CD2AP in the peripheral blood of patients with NS. However, miR‐939‐5p did not show a regulatory effect on the 3′‐untranslated region of CD2AP. The expression levels of specific protein 1 and adenovirus E2 promoter‐binding factor 1, important transcription regulators in the promoter region of CD2AP, were also not affected by microRNA (miR)‐939‐5p. We confirmed that miR‐939‐5p is in the nucleus by fluorescent in situ hybridization and cytoplasmic separation polymerase chain reaction. The promoter plasmid and miR‐939‐5p were cotransfected into HEK‐293 cells, and the luciferase reporter gene assay was used to analyze the promoter activity. We found that miR‐939‐5p binds to a specific sequence in the CD2AP promoter. miR‐939‐5p was confirmed to reduce the recruitment of RNA polymerase II to the CD2AP promoter region by chromatin immunoprecipitation. These findings improve our understanding of the mechanism of miR‐939‐5p in NS and provide potential molecular therapeutic targets for NS. Increased expression of microRNA (miR)‐939‐5p and decreased expression of CD2‐associated protein (CD2AP) is in children with the primary nephrotic syndrome. miR‐939‐5p is indeed in the nucleus. miR‐939‐5p regulates CD2AP expression in the nucleus in a pretranscriptional manner.