Details

Autor(en) / Beteiligte

• Chen, Feng-Feng
• Li, Lei
• Chen, Xiang-Dong
• Zhou, Cheng
• Liao, Da-Qing

Titel

Xylazine Produced Analgesic Effect via Inhibits Hyperpolarization-activated Cyclic Nucleotide-gated Ion Channels Currents

Ist Teil von

• Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban, 2019-01, Vol.50 (1), p.20-26

Ort / Verlag

China

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the analgesic mechanism of xylazine by inhibiting the activation of hyperpolarized cyclic nucleotide-gated (HCN) ion channels. HCN subchannel 1 (HCN1) knockout mice (HCN1 ) and HCN1 wild type mice (HCN1 ) were intraperitoneally injected with physiological saline and xylazine (10, 20, 30, and 40 mg/kg). Mechanical pain test and tail flick test were used to test the analgesic effect of xylazine by using the percentage of the maximal possible effect (%MPE); The control group and test groups of different concentrations of xylazine (12.5, 25, 50, and 100 μmol/L) were set up using HEK 293 cells transfected HCN1 plasmid and HCN subchannel 2 (HCN2) plasmid, respectively. The activated current of hyperpolarized HEK 293 cells expressing HCN1 and HCN2 ion channels and the inhibition rate of xylazine on hyperpolarization-activated currents were recorded using a whole cell patch clamp. The results demonstrated that %MPE of the mechanical pain stimuli test and the thermal radiation stimuli test increased wit