Details

Autor(en) / Beteiligte

• Ying, Jun-Jie
• Zhang, Shun-Li
• Huang, Chao-Yu
• Xu, Lin
• Zhao, Zhe
• Wu, Min
• Sun, Cong

Titel

Algicoccus marinus gen. nov. sp. nov., a marine bacterium isolated from the surface of brown seaweed Laminaria japonica

Ist Teil von

• Archives of microbiology, 2019-09, Vol.201 (7), p.943-950

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• A Gram-staining-negative, strictly aerobic, non-motile, ovoid- to rod-shaped bacterium, designated as HZ20 T , was isolated from the surface of a brown seaweed ( Laminaria japonica ) sample collected from the East China Sea. Colonies are 1.0–2.0 mm in diameter, smooth, circular, convex and yellow after grown on MA at 28 °C for 72 h. The strain was found to grow at 4–50 °C (optimum, 37 °C), pH 5.0–9.5 (optimum, pH 7.0–7.5) and with 0–10% (w/v) NaCl (optimum, 1.0–1.5%). Chemotaxonomic analysis showed ubiquinone-8 as the only quinone, C 17:0 cyclo, C 16:0 , summed feature 8 (C 18:1 ω 7 c and/or C 18:1 ω 6 c ) and summed feature 2 (C 12:0 aldehyde/unknown 10.9525/C 16:1 iso I/C 14:0 3OH) as the major fatty acids (> 5%), and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified amino phospholipid, two unidentified phospholipids, five unidentified glycolipid and two unidentified lipids as the polar lipids. The DNA G + C content was 55.5 mol %. 16S rRNA gene sequences of the isolate showed highest similarities to Bordetella flabilis AU10664 T (97.1%), Parapusillimonas granuli Ch07 T (97.1%), Paracandidimonas soli IMT-305 T (97.1%), Kerstersia gyiorum LMG5906 T (97.0%) and Bordetella sputigena LMG 28641 T (97.0%). The phylogenetic trees using 16S rRNA gene and genome sequences both showed that the strain HZ20 T formed a deep branch separated from other related genera, indicating that it represents a novel species of a novel genus. The calculated average nucleotide identity (ANI) and percent of conserved proteins (POCP) values using genome sequences of strain HZ20 T and related strains also support this conclusion. Based on the phenotypic properties and phylogenetic distinctiveness, we propose strain HZ20 T (= MCCC 1K03465 T  = KCTC 62330 T ) to represent a novel species of a novel genus with the name Algicoccus marinus gen. nov. sp. nov.