Details

Autor(en) / Beteiligte

• Nielsen, Thorbjørn B.
• Thomsen, Rasmus P.
• Mortensen, Michael R.
• Kjems, Jørgen
• Nielsen, Per Franklin
• Nielsen, Thomas E.
• Kodal, Anne Louise B.
• Cló, Emiliano
• Gothelf, Kurt V.

Titel

Peptide‐Directed DNA‐Templated Protein Labelling for The Assembly of a Pseudo‐IgM

Ist Teil von

• Angewandte Chemie (International ed.), 2019-07, Vol.58 (27), p.9068-9072

Auflage

International ed. in English

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The development of methods for conjugation of DNA to proteins is of high relevance for the integration of protein function and DNA structures. Here, we demonstrate that protein‐binding peptides can direct a DNA‐templated reaction, selectively furnishing DNA–protein conjugates with one DNA label. Quantitative conversion of oligonucleotides is achieved at low stoichiometries and the reaction can be performed in complex biological matrixes, such as cell lysates. Further, we have used a star‐like pentameric DNA nanostructure to assemble five DNA–Rituximab conjugates, made by our reported method, into a pseudo‐IgM antibody structure that was subsequently characterized by negative‐stain transmission electron microscopy (nsTEM) analysis. Stick to it: A new method for labelling immunoglobulin G (IgG) with DNA is developed and the resulting DNA–IgG conjugates are exploited to form a IgM‐like nanostructure. This method uses protein‐binding peptides to direct a DNA‐templated reaction, selectively producing DNA–protein conjugates with one DNA label.