Details

Autor(en) / Beteiligte

• Roberts, Jeffrey W.

Titel

Mechanisms of Bacterial Transcription Termination

Ist Teil von

• Journal of molecular biology, 2019-09, Vol.431 (20), p.4030-4039

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2019

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Bacterial transcription termination, described mostly for Escherichia coli, occurs in three recognized ways: intrinsic termination, an activity only of the core RNAP enzyme and transcript sequences that encode an RNA hairpin and terminal uridine-rich segment; termination by the enzyme Rho, an ATP-dependent RNA translocase that releases RNA by forcing uncharacterized structural changes in the elongating complex; and Mfd-dependent termination, the activity of an ATP-dependent DNA translocase that is thought to dissociate the elongation complex by exerting torque on a stalled RNAP. Intrinsic termination can be described in terms of the nucleic acid movements in the process, whereas the enzymatic mechanisms have been illuminated importantly by definitive structural and biochemical analysis of their activity. [Display omitted] •Several modes of nucleic acid movement have been proposed to explain how RNA hairpin formation induces termination.•Both the RNA translocase Rho and DNA translocase Mfd move along nucleic acid toward RNAP, engaging it and forcing dissociation of the elongating complex.