Details

Autor(en) / Beteiligte

• Stosiek, Natalia
• Terebieniec, Agata
• Ząbek, Adam
• Młynarz, Piotr
• Cieśliński, Hubert
• Klimek-Ochab, Magdalena

Titel

N-phosphonomethylglycine utilization by the psychrotolerant yeast Solicoccozyma terricola M 3.1.4

Ist Teil von

• Bioorganic chemistry, 2019-12, Vol.93, p.102866-102866, Article 102866

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• [Display omitted] •S. terricola M 3.1.4. is a novel yeast strain capable of using PMG as a source of nutrients for growth.•PMG degradation regardless of the phosphate cell status has been proven.•Psychrotolerant yeast degrades PMG via GOX pathway. Solicoccozyma terricola M 3.1.4., the yeast strain isolated from soil sample from blueberry cultivation in Miedzyrzec Podlaski in Poland, is capable to split of phosphorus to nitrogen and nitrogen to carbon bonds in N-phosphonomethylglycine (PMG, glyphosate). The biodegradation process proceeds in the phosphate-independent manner. It is the first example of a psychrotolerant yeast strain able to degrade PMG via CN bond cleavage accompanied by AMPA formation and not like in most microorganisms via CP bond disruption followed by the sarcosine pathway. Glyphosate oxidoreductase (GOX) type activity was detected in cell-free extracts prepared from S. terricola M 3.1.4. pregrown on 4 mM PMG as a sole phosphorus and nitrogen source in cultivation medium.