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Responses of two ladybird beetle species (Coleoptera: Coccinellidae) to dietary RNAi
Pest management science, 2019-10, Vol.75 (10), p.2652-2662
Haller, Simone
Widmer, Franco
Siegfried, Blair D
Zhuo, Xuguo
Romeis, Jörg
2019
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Haller, Simone
Widmer, Franco
Siegfried, Blair D
Zhuo, Xuguo
Romeis, Jörg
Titel
Responses of two ladybird beetle species (Coleoptera: Coccinellidae) to dietary RNAi
Ist Teil von
Pest management science, 2019-10, Vol.75 (10), p.2652-2662
Ort / Verlag
Chichester, UK: John Wiley & Sons, Ltd
Erscheinungsjahr
2019
Quelle
MEDLINE
Beschreibungen/Notizen
BACKGROUND One concern with the adoption of RNAi‐based genetically engineered (GE) crops is the potential harm to valued non‐target organisms. Species of Coccinellidae (Coleoptera) are important natural enemies and might be exposed to the insecticidal dsRNA produced by the plant. To assess their susceptibility to dietary RNAi, we fed Adalia bipunctata and Coccinella septempunctata with a dsRNA designed to target the vATPase A of the western corn rootworm, Diabrotica virgifera virgifera (Dvv dsRNA). Specific dsRNAs designed to target the vATPase A of the two ladybird beetle species served as positive controls. RESULTS Our results revealed that both species were sensitive to dietary RNAi when ingesting their own dsRNAs, with C. septempunctata being more sensitive than A. bipunctata. Dvv dsRNA also adversely affected the two ladybird beetles as indicated by a significantly (but marginally) prolonged developmental time for A. bipunctata and a significantly reduced survival rate for C. septempunctata. These results, however, were obtained at Dvv dsRNA concentrations that were orders of magnitude higher than expected to occur in the field. Gene expression analyses confirmed the bioactivity of the dsRNA treatments and the results from the feeding bioassays. These results are consistent with the bioinformatics analyses, which revealed a higher number of 21‐nucleotide‐long matches, a requirement for effective RNAi, of the Dvv dsRNA with the vATPase A of C. septempunctata (34 matches) than with that of A. bipunctata (six matches). CONCLUSION Feeding bioassays revealed that two ladybird species are responsive to dietary RNAi. The two species, however, differed in their sensitivity. © 2019 Society of Chemical Industry Ladybird beetles are sensitive to dietary RNAi and might thus be at risk by insecticidal, RNAi‐based genetically modified crops.
Sprache
Englisch
Identifikatoren
ISSN: 1526-498X
eISSN: 1526-4998
DOI: 10.1002/ps.5370
Titel-ID: cdi_proquest_miscellaneous_2194593213
Format
–
Schlagworte
Adalia bipunctata
,
Animals
,
Beetles
,
Bioassays
,
Bioinformatics
,
Biological activity
,
Coccinella septempunctata
,
Coccinellidae
,
Coleoptera
,
Coleoptera - drug effects
,
Coleoptera - growth & development
,
Coleoptera - physiology
,
Corn
,
Diabrotica virgifera virgifera
,
Diet
,
Double-stranded RNA
,
dsRNA
,
environmental risk assessment
,
Feeding
,
Gene expression
,
Genetic engineering
,
Insect Control
,
Moths
,
Natural enemies
,
non‐target effects
,
Nucleotides
,
Organic chemistry
,
RNA Interference - physiology
,
RNA, Double-Stranded - toxicity
,
RNA-mediated interference
,
Species
,
Species Specificity
,
vATPase A
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