Biochemical and biophysical research communications, 2019-04, Vol.512 (2), p.331-337
2019
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- Autor(en) / Beteiligte
- Titel
- MicroRNA-107 induces cell cycle arrests by directly targeting cyclin E1 in ovarian cancer
- Ist Teil von
- Biochemical and biophysical research communications, 2019-04, Vol.512 (2), p.331-337
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Deregulated expression of microRNAs plays oncogenic or anti-oncogenic roles in various cancers. However, expression of miR-107 was not consistent among several types of cancer, and the effect of miR-107 in ovarian cancer remains unclear. In this study, we found that expression miR-107 was significantly decreased in ovarian cancer patients and in cell lines. Ectopic expression of miR-107 suppressed cell proliferation and G1 phase to S transition of cell cycle, and was associated with downregulation of cyclin E1 (CCNE1) expression. Mechanistically, CCNE1 was confirmed to be a direct target of miR-107 through the dual-luciferase reporter assay. Knockdown of CCNE1 dramatically impeded cell cycle in G1/S phase transition similarly as miR-107 overexpression did. In addition, overexpression of CCNE1 reversed the inhibition of cell proliferation induced by miR-107 overexpression. Finally, miR-107 had anti-cancer potential by suppressing tumor initiation and progression in vivo. Our finding indicates that miR-107 serves as a tumor suppressor by decreasing CCNE1 expression levels, which may provide potential therapeutic strategies in ovarian cancer treatment. •Expression of miR-107 was decreased in ovarian cancer.•Elevated miR-107 suppressed cell proliferation and G1 phase to S transition of cell cycle.•Cyclin E1 was confirmed to be a direct target of miR-107.•miR-107 had anti-cancer potential by suppressing tumor initiation and progression in vivo.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-291XeISSN: 1090-2104DOI: 10.1016/j.bbrc.2019.03.009Titel-ID: cdi_proquest_miscellaneous_2194141077
- Format
- –
- Schlagworte
- Animals, CCNE1, Cell cycle, Cell Line, Tumor, Cell Proliferation - genetics, Cyclin E - antagonists & inhibitors, Cyclin E - genetics, Cyclin E - metabolism, Down-Regulation, Female, G1 Phase Cell Cycle Checkpoints - genetics, Gene Expression Regulation, Neoplastic, Gene Knockdown Techniques, Genes, Tumor Suppressor, Heterografts, Humans, Mice, Mice, Nude, MicroRNAs - genetics, MicroRNAs - metabolism, miR-107, Oncogene Proteins - antagonists & inhibitors, Oncogene Proteins - genetics, Oncogene Proteins - metabolism, Ovarian cancer, Ovarian Neoplasms - genetics, Ovarian Neoplasms - metabolism, Ovarian Neoplasms - pathology, Phosphorylation, Retinoblastoma Protein - metabolism