Details

Autor(en) / Beteiligte

• da Silva, Marcelo Henrique Matias
• Moises, Raiza Nara Cunha
• Alves, Brenda Elen Bizerra
• Pereira, Hannaly Wana Bezerra
• de Paiva, Anne Aline Pereira
• Morais, Ingryd Câmara
• Nascimento, Yasmim Mesquita
• Monteiro, Joelma Dantas
• de Souto, Janeusa Trindade
• Nascimento, Manuela Sales Lima
• de Araújo, Josélio Maria Galvão
• da Guedes, Paulo Marcos Matta
• Fernandes, José Veríssimo

Titel

Innate immune response in patients with acute Zika virus infection

Ist Teil von

• Medical microbiology and immunology, 2019-12, Vol.208 (6), p.703-714

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2019

Quelle

SpringerLink

Beschreibungen/Notizen

• Innate immunity receptors (Toll-like receptors/TLRs and RIG-like receptors/RLRs) are important for the initial recognition of Zika virus (ZIKV), modulation of protective immune response, and IFN-α and IFN-β production. Immunological mechanisms involved in protection or pathology during ZIKV infection have not yet been determined. In this study, we evaluated the mRNA expression of innate immune receptors (TLR3, TLR7, TLR8, TLR9, melanoma differentiation-associated protein 5/MDA-5, and retinoic acid inducible gene/RIG-1), its adapter molecules ( Myeloid Differentiation Primary Response Gene 88 /Myd88, Toll / IL-1 Receptor Domain-Containing Adaptor-Inducing IFN-β/TRIF), and cytokines (IL-6, IL-12, TNF-α, IFN-α, IFN-β, and IFN-γ) in the acute phase of patients infected by ZIKV using real-time PCR in peripheral blood. Patients with acute ZIKV infection had high expression of TLR3, IFN-α, IFN-β, and IFN-γ when compared to healthy controls. In addition, there was a positive correlation between TLR3 expression compared to IFN-α and IFN-β. Moreover, viral load is positively correlated with TLR8, RIG-1, MDA-5, IFN-α, and IFN-β. On the other hand, patients infected by ZIKV showed reduced expression of RIG-1, TLR8, Myd88, and TNF-α molecules, which are also involved in antiviral immunity. Similar expressions of TLR7, TLR9, MDA-5, TRIF, IL-6, and IL-12 were observed between the group of patients infected with ZIKV and control subjects. Our results indicate that acute infection (up to 5 days after the onset of symptoms) by ZIKV in patients induces the high mRNA expression of TLR3 correlated to high expression of IFN-γ, IFN-α, and IFN-β, even though the high viral load is correlated to high expression of TLR8, RIG-1, MDA-5, IFN-α, and IFN-β in ZIKV patients.