Details

Autor(en) / Beteiligte

• Morimoto, Takeshi
• Chiu, Liang-da
• Kanda, Hiroyuki
• Kawagoe, Hiroyuki
• Ozawa, Takeaki
• Nakamura, Makoto
• Nishida, Kohji
• Fujita, Katsumasa
• Fujikado, Takashi

Titel

Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction

Ist Teil von

• Analyst (London), 2019-04, Vol.144 (8), p.2531-2540

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• Mitochondrial activity is a widely used criterion to judge the metabolic condition of a living specimen. Numerous methods have been developed for related analyses, including the detection of O2 consumption, trans-membrane potential, and ATP production. In this study, we demonstrate that the redox state of cytochromes can serve as a sensitive mitochondrial activity indicator in glutamate-stressed neuronal cells. Mitochondrial dysfunction was detected by Raman imaging as early as 30 min after glutamate-stress induction. By comparing this result with other commonly used mitochondrial function assays, we found Raman imaging has a similar sensitivity to ATP production and trans-membrane potential assays. Other viability tests, such as MTT assay and ROS production tests, showed a slower response than our method. A thorough understanding of cytochrome dynamics with our new method will help establish Raman spectroscopy as a competitive clinical diagnosis tool for neurodegenerative diseases involving mitochondrial dysfunction.