Details

Autor(en) / Beteiligte

• Shakeri, Abolfazl
• D’Urso, Gilda
• Taghizadeh, Seyedeh Faezeh
• Piacente, Sonia
• Norouzi, Saeed
• Soheili, Vahid
• Asili, Javad
• Salarbashi, Davoud

Titel

LC-ESI/LTQOrbitrap/MS/MS and GC–MS profiling of Stachys parviflora L. and evaluation of its biological activities

Ist Teil von

• Journal of pharmaceutical and biomedical analysis, 2019-05, Vol.168, p.209-216

Ort / Verlag

England: Elsevier B.V

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •Antimicrobial, antioxidant, and cytotoxic activities of the methanolic extract/essential oil of S. parviflora L. were tested.•Eighty-seven compounds were characterized in the EO of S. parviflora using GC–MS.•30 metabolites were identified in S. parviflora methanolic extract, mainly pertaining to phenolic compounds.•Strong antifungal activity has demonstrated by the EO of S. parviflora against C. albicans (MFC = 0.28 μg/mL). The use of some Stachys genus as herbal remedies is known and the aerial parts have a pharmaceutical interest, being used in Anatolia and Iran as wild tea. In this study, chemical composition, antimicrobial, antioxidant, and antiproliferative activities of the methanolic extract and essential oil (EO) of Stachys parviflora L. (S. parviflora) were evaluated. Qualitative analysis of metabolites of S. parviflora methanolic extract was studied using liquid chromatography coupled to high resolution mass spectrometry (LC-ESI/LTQOrbitrap/MS), evidencing the presence of phenolic acids and flavonoids derivatives. The EO was analyzed using gas chromatography coupled to mass spectrometry (GC/MS). Eighty-seven compounds were characterized in the EO of S. parviflora, of which α-terpenyl acetate (23.6%), β-caryophyllene (16.8%), bicyclogermacrene (9.3%), spathulenol (4.9%) and α-pinene (4.2%) were found to be the major components. The highest antimicrobial effect of EO was found to S. aureus and B. cereus (MIC = 0.01 μg/ml), while the highest activity of extract was against B. cereus (MIC = 125 μg/ml). The methanolic extract exhibited strong antioxidant activity in DPPH (IC50 = 76.87 μg/ml) and β-carotene/linoleic acid assay (BCB, IC50 = 188.47 μg/ml) methods. Furthermore, in vitro cytotoxicity evaluation against three cell lines namely human ovarian carcinoma (A2780), human colon carcinoma (HCT), and mouse melanoma cell line (B16F10), showed an anti-proliferative activity of the EO ranging from IC50 value 30.95 μg/ml to 16.55 μg/ml. The results from this study have demonstrated the promising cytotoxic, antibacterial, and antifungal properties of S. parviflora, which could have wide potential applications in food and pharmaceutical industries.