Details

Autor(en) / Beteiligte

• Zhu, Dan
• Hou, Lixia
• Xiao, Peilian
• Guo, Yang
• Deyholos, Michael K.
• Liu, Xin

Titel

VvWRKY30, a grape WRKY transcription factor, plays a positive regulatory role under salinity stress

Ist Teil von

• Plant science (Limerick), 2019-03, Vol.280, p.132-142

Ort / Verlag

Ireland: Elsevier B.V

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• •The transcriptional activation activity and nuclear localization of VvWRKY30 were confirmed.•VvWRKY30 showed key transcriptional activity domain at the N-terminal and specifically binds to the W-BOX.•The expression of VvWRKY30 was induced by salt as well as stress signaling molecules H2S and H2O2.•Overexpression of VvWRKY30 increased plant salt stress resistance by regulating reactive oxygen species-scavenging and osmoticum accumulation. High salinity severely inhibits the growth and productivity of grape plants. However, knowledge of salt-stress regulation remains limited in WRKY members of grapes. Here, we isolated a novel VvWRKY30 gene from Vitis vinifera L. and studied its role in salt-stress resistance. The VvWRKY30 protein fused with green fluorescent protein localized to the nucleus and the transcriptional activation activity of VvWRKY30 was confirmed in yeast. Moreover, VvWRKY30 showed key transcriptional activity domain at the N-terminal and specifically binds to the W-BOX. VvWRKY30 showed the highest expression in the shoot tip and functional leaves of grape plants. VvWRKY30 expression was induced by salt as well as stress signaling molecules H2S and H2O2. Overexpression of VvWRKY30 in Arabidopsis increased its resistance to salt stress at different stages of growth. Under salinity stress, VvWRKY30 overexpressing lines had higher antioxidant activities and lower reactive oxygen species contents. Soluble sugar and proline concentrations also increased in VvWRKY30 overexpressing lines in the presence of NaCl. In addition, the transcription of genes related to antioxidant biosynthesis, glyco-metabolism and proline biosynthesis increased in the VvWRKY30 overexpressing lines. Taken together, this study confirmed the important role of VvWRKY30 in increasing salt stress resistance by regulating reactive oxygen species-scavenging and the accumulation of osmoticum.