Details

Autor(en) / Beteiligte

• Favela-Candia, Alejandro
• Téllez-Valencia, Alfredo
• Campos-Almazán, Mara
• Sierra-Campos, Erick
• Valdez-Solana, Mónica
• Oria-Hernández, Jesús
• Castillo-Villanueva, Adriana
• Nájera, Hugo
• Avitia-Domínguez, Claudia

Titel

Biochemical, Kinetic, and Computational Structural Characterization of Shikimate Kinase from Methicillin-Resistant Staphylococcus aureus

Ist Teil von

• Molecular biotechnology, 2019-04, Vol.61 (4), p.274-285

Ort / Verlag

New York: Springer US

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• One of the most widespread pathogens worldwide is methicillin-resistant Staphylococcus aureus , a bacterium that provokes severe life-threatening illnesses both in hospitals and in the community. The principal challenge lies in the resistance of MRSA to current treatments, which encourages the study of different molecular targets that could be used to develop new drugs against this infectious agent. With this goal, a detailed characterization of shikimate kinase from this microorganism (SaSK) is described. The results showed that SaSK has a K m of 0.153 and 224 µM for shikimate and ATP, respectively, and a global reaction rate of 13.4 µmol/min/mg; it is suggested that SaSK utilizes the Bi–Bi Ping Pong reaction mechanism. Furthermore, the physicochemical data indicated that SaSK is an unstable, hydrophilic, and acidic protein. Finally, structural information showed that SaSK presented folding that is typical of its homologous counterparts and contains the typical domains of this family of proteins. Amino acids that have been shown to be important for SaSK protein function are conserved. Therefore, this study provides fundamental information that may aid in the design of inhibitors that could be used to develop new antibacterial agents.