Details

Autor(en) / Beteiligte

• Mamoori, Afraa
• Wahab, Riajul
• Vider, Jelena
• Gopalan, Vinod
• Lam, Alfred King-yin

Titel

The tumour suppressor effects and regulation of cancer stem cells by macrophage migration inhibitory factor targeted miR-451 in colon cancer

Ist Teil von

• Gene, 2019-05, Vol.697, p.165-174

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• This study aimed to investigate the impact of miR-451 on the biological behaviours of colon cancer cells along with its targets interactions. The levels of miR-451 were tested in colon cancer cell lines (SW480 and SW48). Multiple functional and immunological assays were performed to analyse miR-451 induced growth changes in-vitro and downstream effects on target proteins. Overexpression of miR-451 in colon cancer cells led to reduced cell proliferation, increased apoptosis and decrease accumulation of the cells at the G0/G1 phase of the cell cycle. In addition, a significant increase in the number of the cells was noted in the G2-M phase of cell cycle. Moreover, miR-451 reduced the expression of Oct-4, Sox-2 and Snail indicating its role in stem cell and epithelial-mesenchymal transition (EMT) regulation. An inverse correlation between miR-451 and macrophage migration inhibitory protein (MIF) protein expression occurred in colon cancer cells. Furthermore, restoration the level of miR-451 in colon cancer cells inhibits tumour spheres formation. miR-451 has tumour suppressor effects in vitro, which can inhibit the cancer-related signalling pathways in colon cancer. •miR-451 has tumour suppressor effects in vitro in colon cancer•miR-451 reduced the expression of Oct-4, Sox-2 and Snail in colon cancer.•Inverse correlation between miR-451 and MIF expression in colon cancer.•Restoration the level of miR-451 in colon cancer inhibits tumour spheres formation.