Details

Autor(en) / Beteiligte

• Porras-Domínguez, Jaime R.
• Rodríguez-Alegría, María Elena
• Miranda, Alfonso
• Alvarez Berber, Laura Patricia
• Edmundo, Castillo
• López Munguía, Agustín

Titel

Frucooligosaccharides purification: Complexing simple sugars with phenylboronic acid

Ist Teil von

• Food chemistry, 2019-07, Vol.285, p.204-212

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• •FOS can be purified by a selective chemical method using phenyl boronic acid.•PBAc react selectively with glucose and fructose to change their polarity.•Only the free fructose pyranose conformer can react with PBAc.•Free furanose and pyranose α-glucose conformers may react with PBAc.•NMR reveals five different glucose-PBAc extraction complexes. Prebiotic fructooligosaccharides (FOS) are currently obtained by enzymatic reaction with fructosyltransferases (FTFs) using sucrose as both donor and acceptor. In these reactions glucose results as the most abundant by-product, arising from each fructosyl transfer event and, together with fructose, because of the inherent hydrolytic activity of the FTFs. As FOS are mainly used as prebiotic in nutraceutical foods, the reduction or total elimination of monosaccharides is required. In this work the selective elimination of monosaccharides from a synthetic FOS mixture was achieved through the selective complexation of glucose and fructose with phenyl boronic acid (PBAc) followed by ethyl-acetate extraction. The process was applied to a complex mixture of FOS obtained in an enzymatic synthesis reaction containing 40% glucose, 15.8% fructose and 35% of FOS, elimination of the sugars was achieved through 3:1 molar reactions, resulting in a levan-type FOS product with 97% purity.