Details

Autor(en) / Beteiligte

• Yang, Li‐yun
• Shan, Ya‐min
• Zhang, Yi
• Zhou, En‐hui
• Chen, Xiao‐ping
• Zhang, Hao

Titel

Aurora kinase A induces chemotherapy resistance through revival of dormant cells in laryngeal squamous cell carcinoma

Ist Teil von

• Head & neck, 2019-07, Vol.41 (7), p.2239-2248

Ort / Verlag

Hoboken, USA: John Wiley & Sons, Inc

Erscheinungsjahr

2019

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Background Chemotherapy resistance was an important tumor metastasis mechanism. Methods Cell Counting Kit‐8 assay and plate colony formation assay were applied to examine the proliferation of laryngeal squamous cell carcinoma (LSCC). Immunofluorescent staining and Western blotting were carried out to show the expression of related proteins. Wound healing, migration, and invasion assays were used to examine the mobility, migration, and invasion of LSCC. Results Downregulated Aurora kinase A (AURKA) increased chemotherapy sensitivity and reduced the ability of mobility, migration, and invasion of Hep2 cells, while upregulated AURKA possessed opposite results. Hep2/5‐Fu cells possessed dormancy‐like properties and upregulated AURKA in Hep2/5‐Fu cells (Hep2/5‐Fu/AURKA cells) revived dormant state. Furthermore, Erk1/2 was restrained in Hep2/5‐Fu cells and activated in Hep2/5‐Fu/AURKA cells. Moreover, Erk1/2 accelerated the ability of mobility, migration, and invasion in Hep2/5‐Fu/AURKA cells. Conclusion AURKA activated dormant state to induce chemotherapy resistance and promoted metastasis of LSCC through Erk1/2 pathway.