Details

Autor(en) / Beteiligte

• Ge, J
• Li, Y J
• Liu, A Q
• Huang, W Y
• Yang, F
• Ma, L
• Zhai, H H

Titel

Changes of intestinal mucosal barrier in mice with chronic ulcerative colitis

Ist Teil von

• Zhong hua yi xue za zhi, 2018-12, Vol.98 (48), p.3950-3953

Ort / Verlag

China

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• To study the damage and mechanism of intestinal mucosal barrier function in mice with ulcerative colitis induced by Dextran sulphate sodium (DSS). Mice models of chronic ulcerative colitis induced by DSS were established. The mice were completely randomized into normal control group and DSS group, 25 mice in each group. The body weight and colon length of the mice were monitored. The pathological examination of colon tissue was confirmed the success of the model and assessed the integrity of the colonic mucosal barrier; Evan's Blue's intestinal permeability analysis assessed the function of colon mucosal barrier; immunofluorescence staining and Western blot were used to evaluate the expression of intestinal mucosal barrier integrity-related proteins. Compared with the normal control group, the DSS group had lower body weight [(25.6±0.7)g vs (23.5±0.7)g, =2.14, <0.05], and the colon length was shorter [(7.3±0.4)cm vs (5.6±0.2)cm, =3.975, <0.001]; colonic pathological results showed that the intestinal mucosa b