Details

Autor(en) / Beteiligte

• Razavi, Morteza
• Farrokhi, Vahid
• Yip, Richard
• Anderson, N Leigh
• Pearson, Terry W
• Neubert, Hendrik

Titel

Measuring the Turnover Rate of Clinically Important Plasma Proteins using an Automated SISCAPA Workflow

Ist Teil von

• Clinical chemistry (Baltimore, Md.), 2019-03, Vol.65 (3), p.492-494

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

2019

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Clinical Pulse-Labeling of Plasma Proteins and Sample Analysis Recently, a unique pulse-chase study was described in which blood samples collected from human subjects infused with stable isotope labeled leucine were analyzed using an immunoaffinity mass-spectrometric approach to determine protein turnover rates (3). Importantly, of the 24 proteins, 19 had turnover rates shorter than 5 days, and of those, 8 proteins approximately 1 day or less. [...]for proteins with shorter half-lives, accurate measurement of variations in protein con- centration on a personal level would require a daily sampling frequency. Author Contributions: Allauthors confirmed they have contributed to the intellectual content of this paper and have met the following 4 requirements: (a) significant contributions to the conception and design, acquisition of data, or analysis and interpretation ofdata; (b) drafting or revising the article for intellectual content; (c) final approval ofthe published article; and (d) agreement to be accountable for all aspects ofthe article thus ensuringthatquestions related to the accuracy or integrity ofanypart ofthe article are appropriately investigated and resolved. 1 SISCAPA Assay Technologies, Inc. Washington, DC 2 Biomedicine Design, Worldwide Research & Development, Pfizer Inc. Andover, MA * Address correspondence to this author at: SISCAPA Assay Technologies, Inc. Box 53309 Washington, DC 20009 Fax 250-721-8855 E-mail mrazavi@siscapa.com † M. Razavi and V. Farrokhi contributed equally to this work.