Details

Autor(en) / Beteiligte

• Hasan, Sadat
• Geissler, David
• Wink, Konstantin
• Hagen, Axel
• Heiland, Josef J
• Belder, Detlev

Titel

Fluorescence lifetime-activated droplet sorting in microfluidic chip systems

Ist Teil von

• Lab on a chip, 2019-01, Vol.19 (3), p.403-409

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• We present a highly efficient microfluidic fluorescence lifetime-activated droplet sorting (FLADS) approach as a novel technology for droplet manipulation in lab-on-a-chip devices. In a proof-of-concept study, we successfully applied the approach to sort droplets containing two different fluorescent compounds on the basis of their corresponding fluorescence lifetime. Towards this end, a technical set-up was developed enabling on-the-fly fluorescence lifetime determination of passing droplets. The herein developed LabVIEW program enabled fast triggering of a downstream dielectrophoretic force sorting functionality depending on average fluorescence lifetimes of individual droplets. The approach worked reliably at individual substrate concentrations from 1 nM to 1 mM. This not only allowed reliable sorting of droplets containing species with different fluorescence lifetimes but also enabled differentiation of mixtures in individual droplets.