Details

Autor(en) / Beteiligte

• Boda, Attila
• Lőrincz, Péter
• Takáts, Szabolcs
• Csizmadia, Tamás
• Tóth, Sarolta
• Kovács, Attila L.
• Juhász, Gábor

Titel

Drosophila Arl8 is a general positive regulator of lysosomal fusion events

Ist Teil von

• Biochimica et biophysica acta. Molecular cell research, 2019-04, Vol.1866 (4), p.533-544

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2019

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The small GTPase Arl8 is known to be involved in the periphery-directed motility of lysosomes. However, the overall importance of moving these vesicles is still poorly understood. Here we show that Drosophila Arl8 is required not only for the proper distribution of lysosomes, but also for autophagosome-lysosome fusion in starved fat cells, endosome-lysosome fusion in garland nephrocytes, and developmentally programmed secretory granule degradation (crinophagy) in salivary gland cells. Moreover, proper Arl8 localization to lysosomes depends on the shared subunits of the BLOC-1 and BORC complexes, which also promote autophagy and crinophagy. In conclusion, we demonstrate that Arl8 is responsible not only for positioning lysosomes but also acts as a general lysosomal fusion factor. •Loss of Arl8 causes perinuclear clustering of (auto)lysosomes in fat cells.•Arl8 mediates lysosomal fusions with autophagosomes, endosomes and glue granules.•Shared BLOC-1/BORC complex subunits promote Arl8 function, autophagy and crinophagy.