Details

Autor(en) / Beteiligte

• Akichika, Shinichiro
• Hirano, Seiichi
• Shichino, Yuichi
• Suzuki, Takeo
• Nishimasu, Hiroshi
• Ishitani, Ryuichiro
• Sugita, Ai
• Hirose, Yutaka
• Iwasaki, Shintaro
• Nureki, Osamu
• Suzuki, Tsutomu

Titel

Cap-specific terminal N 6 -methylation of RNA by an RNA polymerase II-associated methyltransferase

Ist Teil von

• Science (American Association for the Advancement of Science), 2019-01, Vol.363 (6423)

Ort / Verlag

United States

Erscheinungsjahr

2019

Link zum Volltext

Quelle

American Association for the Advancement of Science

Beschreibungen/Notizen

• -methyladenosine (m A), a major modification of messenger RNAs (mRNAs), plays critical roles in RNA metabolism and function. In addition to the internal m A, , 2'- -dimethyladenosine (m Am) is present at the transcription start nucleotide of capped mRNAs in vertebrates. However, its biogenesis and functional role remain elusive. Using a reverse genetics approach, we identified PCIF1, a factor that interacts with the serine-5-phosphorylated carboxyl-terminal domain of RNA polymerase II, as a cap-specific adenosine methyltransferase (CAPAM) responsible for -methylation of m Am. The crystal structure of CAPAM in complex with substrates revealed the molecular basis of cap-specific m A formation. A transcriptome-wide analysis revealed that -methylation of m Am promotes the translation of capped mRNAs. Thus, a cap-specific m A writer promotes translation of mRNAs starting from m Am.