Details

Autor(en) / Beteiligte

• Souza, Israel D.
• Hantao, Leandro W.
• Queiroz, Maria Eugênia C.

Titel

Polymeric ionic liquid open tubular capillary column for on-line in-tube SPME coupled with UHPLC-MS/MS to determine endocannabinoids in plasma samples

Ist Teil von

• Analytica chimica acta, 2019-01, Vol.1045, p.108-116

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• This manuscript describes the development of wall-coated open tubular capillary column with polymeric ionic liquids (PILs) for on-line in-tube solid phase microextraction coupled with ultra high-performance liquid chromatography tandem mass spectrometry (in-tube SPME/UHPLC-MS/MS) to determine anandamide (AEA) and 2-arachidonoyl glycerol (2 A G) in plasma samples. Selective PILs were synthetized from the [VC6IM][Cl], [VC16IM][Br], and [(VIM)2C10]2 [Br] - ionic liquids - by in-situ thermal-initiated polymerization in a fused silica capillary column for in-tube SPME. The synthesis procedure was optimized, and the capillary columns were characterized using spectroscopic and chromatography techniques. The chemically bonded and cross-linked PIL-based sorbent phase (thickness coating: 1.7 μm) presented high chemical and mechanical stability. Among the sorbents evaluated, the PIL-based capillary, [VC16IM][Br]/[(VIM)2C10]2 [Br] presented the best performance with a sorption capacity of 37,311 ng cm−3 and 48,307 ng cm−3 for AEA and 2 A G, respectively. This capillary was reused more than ninety times without significant changes in extraction efficiency. The in-tube SPME-UHPLC-MS/MS method presented a linear range from 0.1 ng mL−1 to 100 ng mL−1 for AEA, and from 0.05 ng mL−1 to 100 ng mL−1 for 2 A G, with coefficients of determination higher than 0.99, p-value for Lack-of-fit test higher than 0.05 (α of 0.05), precision with coefficient of variation (CV) values ranging from 1.6 to 14.0% and accuracy with relative standard deviation (RSD) values from −19.6% to 13.2%. This method was successfully applied to determine AEA and 2 A G in plasma patients with Parkinson's disease. The concentrations in these plasma samples ranged from 0.14 to 0.46 ng mL−1 for AEA and from <0.05 ng mL−1 to 0.51 ng mL−1 for 2-AG. [Display omitted] •Polymeric ionic liquid monomers (PIL) and PIL cross-linker was synthesized.•Selective PIL-based wall-coated open tubular capillary columns were synthesized.•PIL-based wall-coated open tubular capillary was used as a sorbent phase.•PIL capillary was used in on-line in-tube SPME/UHPLC-MS/MS method.•The method was applied to determine endocannabinoids in plasma samples.