Details

Autor(en) / Beteiligte

• Liang, Bin
• Ma, Jincai
• Cai, Weiwei
• Li, Zhiling
• Liu, Wenzong
• Qi, Mengyuan
• Zhao, Youkang
• Ma, Xiaodan
• Deng, Ye
• Wang, Aijie
• Zhou, Jizhong

Titel

Response of chloramphenicol-reducing biocathode resistome to continuous electrical stimulation

Ist Teil von

• Water research (Oxford), 2019-01, Vol.148, p.398-406

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Understanding the fate of overall antibiotic resistance genes (ARGs) during the biological treatment of antibiotic containing wastewater is a central issue for the water ecological safety assessment. Although the microbial electrode-respiration based biotransformation process could significantly detoxify some antibiotic contaminants, e.g. chloramphenicol (CAP), the response of CAP-reducing biocathode microbiome and resistome to continuous electrical stimulation, especially ARGs network interactions, are poorly understood. Here, using highthroughput functional gene array (GeoChip v4.6) and Illumina 16S rRNA gene MiSeq sequencing, the structure, composition, diversity and network interactions of CAP-reducing biocathode microbiome and resistome in response to continuous electrical stimulation were investigated. Our results indicate that the CAP bioelectroreduction process could significantly accelerate the elimination of antibacterial activity of CAP during CAP-containing wastewater treatment compared to the pure bioreduction process. Continuous electrical stimulation could obviously alter both the microbiome and resistome structures and consistently decrease the phylogenetic, functional and overall ARGs diversity and network complexity within the CAP-reducing biofilms. The relative abundances of overall ARGs and specific CAP resistance related major facilitator superfamily (MFS) transporter genes were significantly negatively correlated with the reduction efficiency of CAP to inactive antibacterial product AMCl (partially dechlorinated aromatic amine), which may reduce the ecological risk associated with the evolution of multidrug-resistant bacteria and ARGs during antibiotic-containing wastewater treatment process. This study offers new insights into the response of an antibiotic reducing biocathode resistome to continuous electrical stimulation and provides useful information on the assessment of overall ARGs risk for the bioelectrochemical treatment of antibiotic contaminants. [Display omitted] •Electrical stimulation enhanced chloramphenicol (CAP) bioreduction process.•Electrical stimulation altered the CAP-reducing biocathode resistome structure.•Electrical stimulation decreased overall ARGs abundance and network complexity.•MFS transporter abundance was negatively correlated with CAP reduction efficiency.