Details

Autor(en) / Beteiligte

• Hendrix, Chris
• Rosemeyer, Helmut
• Verheggen, Ilse
• Van Aerschot, Arthur
• Seela, Frank
• Herdewijn, Piet

Titel

1′, 5′ -Anhydrohexitol Oligonucleotides: Synthesis, Base Pairing and Recognition by Regular Oligodeoxyribonucleotides and Oligoribonucleotides

Ist Teil von

• Chemistry : a European journal, 1997-01, Vol.3 (1), p.110-120

Ort / Verlag

Weinheim: WILEY-VCH Verlag

Erscheinungsjahr

1997

Quelle

Wiley-Blackwell subscription journals

Beschreibungen/Notizen

• Oligonucleotides constructed of 1′, 5′‐anhydrohexitol nucleoside building blocks (hexitol nucleic acids, HNA) are completely stable towards 3′‐exonuclease and form very stable self‐complementary duplexes as well as sequence‐selective stable duplexes with the natural DNA and RNA. Triple‐helix formation has also been observed. These hybridisation characteristics are highly dependent on the base sequence and the experimental conditions. When using a phosphate buffer containing 0.1M NaCl, a homopurine HNA dodecamer gives a δTm of +3.0 °C/ base pair with RNA as complement. These oligomers may therefore be of considerable interest as antisense constructs. Complete stability towards 3′‐exonuclease is observed for oligonucleotides constructed from 1′,5′‐anhydrohexitol nucleoside building blocks (hexitol nucleic acids, HNA; see Figure). HNAs form very stable self‐complementary duplexes as well as sequence‐selective stable duplexes with the corresponding natural DNA and RNA, depending on the base sequence and the experimental conditions.