Details

Autor(en) / Beteiligte

• Rahmani, Nanik
• Kahar, Prihardi
• Lisdiyanti, Puspita
• Lee, Jaemin
• Yopi
• Prasetya, Bambang
• Ogino, Chiaki
• Kondo, Akihiko

Titel

GH-10 and GH-11 Endo-1,4-β-xylanase enzymes from Kitasatospora sp. produce xylose and xylooligosaccharides from sugarcane bagasse with no xylose inhibition

Ist Teil von

• Bioresource technology, 2019-01, Vol.272, p.315-325

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• [Display omitted] •Novel GH-10, GH-11 endoxylanases from Kitasatospora were expressed in S. lividans.•The enzyme showed high specific activity, thermostable and broad range pH activity.•High hydrolysis on xylan-based substrates and on extracted xylan from bagasse.•Kitasatospora can produce enzymes capable for xylose and XOS production. A novel strategy for the low-cost, high-yield co-production of xylose and xylooligosaccharides together with no xylose inhibition was developed using a novel heterologous expression of XYN10Ks_480 endo-1,4-β-xylanase with a ricin-type β-trefoil type of domain and XYN11Ks_480 endo-1,4-β-xylanase with a CBM 2 superfamily from the Kitasatospora sp in an actinomycetes expression system. Xylose is the main building block for hemicellulose xylan. Our findings demonstrated high levels of expression and catalytic activity for XYN10Ks_480 during hydrolysis of the extracted xylan of bagasse, and three types of xylan-based substrates were used to produce xylose and xylooligosaccharides. However, hydrolysis by XYN11Ks_480 produced xylooligosaccharides without xylose formation. This study demonstrated how integrating sodium hypochlorite-extracted xylan and enzymatic hydrolysis could provide an alternative strategy for the generation of XOS from lignocellulosic material.