Details

Autor(en) / Beteiligte

• Seo, Min-Goo
• Ouh, In-Ohk
• Lee, Haeseung
• Geraldino, Paul John L.
• Rhee, Man Hee
• Kwon, Oh-Deog
• Kwak, Dongmi

Titel

Differential identification of Anaplasma in cattle and potential of cattle to serve as reservoirs of Anaplasma capra, an emerging tick-borne zoonotic pathogen

Ist Teil von

• Veterinary microbiology, 2018-11, Vol.226, p.15-22

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• •Differential identification of Anaplasma spp. in 1219 South Korean cattle by PCR and RFLP.•Anaplasma bovis and A. capra were detected in 1.0% and 0.4% using 16S rRNA and multilocus genes.•This study suggests the potential of cattle to serve as reservoirs of A. capra.•The occurrence of a new emerging A. capra strain with public health and veterinary significance in Korean cattle. Bovine anaplasmosis is a tick-borne, infectious, non-contagious disease caused by Anaplasma marginale, A. centrale, A. bovis, and zoonotic A. phagocytophilum. Recently, Anaplasma capra detected in goats was identified as a novel zoonotic pathogen. To determine whether A. capra can infect bovines, we used PCR to differentially diagnose Anaplasma spp. in 1219 South Korean cattle by performing multilocus gene typing and restriction fragment length polymorphism (RFLP). Nucleotide sequencing and phylogenetic analysis detected the 16S rRNA gene of A. bovis and four genes from A. capra in 12 (1.0%) and five (0.4%) cattle, respectively. Supplementary discrimination between A. bovis and A. capra was accomplished by RFLP. The 16S rRNA, msp4, groEL, and gltA genes of A. capra identified in this study had much lower degrees of identity to those in A. centrale and other Anaplasma spp. A. phagocytophilum was not detected in any of the tested cattle. Although the prevalence was low, this study suggests the potential of cattle to serve as reservoirs of A. capra. Thus, further studies are needed to clarify the pathogenesis of A. capra in cattle and its possible involvement in transmission to humans.