Euphytica, 2010-02, Vol.171 (3), p.327-335, Article 327
2010
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Details
- Autor(en) / Beteiligte
- Titel
- Identification of DNA markers linked to an induced mutated gene conferring resistance to powdery mildew in pea (Pisum sativum L.)
- Ist Teil von
- Euphytica, 2010-02, Vol.171 (3), p.327-335, Article 327
- Ort / Verlag
- Dordrecht: Dordrecht : Springer Netherlands
- Erscheinungsjahr
- 2010
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- We have recently induced two powdery mildew (Erysiphe pisi Syd) resistant mutants in Pisum sativum L. via ethylnitrosourea (ENU) mutagenesis. Both mutations (er1mut1 and er1mut2) affected the same locus er1 that determines most of the identified natural sources of powdery mildew resistance (PMR) in this crop. The mutated gene er1mut2 was mapped to a linkage group of 16 DNA markers combining three main strategies: near isogenic lines (NILs) analysis, bulked segregant analysis and genetic mapping of randomly identified polymorphic markers, together with three DNA-markers techniques: ISSR, RAPDs and AFLPs. Markers located closer to the PMR locus, OPO06₁₁₀₀y (0.5 cM), OPT06₄₈₀ (3.3 cM) and AGG/CAA₁₂₅ (5.5 cM), were cloned and converted into SCAR markers. Markers AH1R₈₅₀ and AHR₉₂₀y were found to be allelic and converted into the co-dominant marker ScAH1 (16.3 cM). Two previously known DNA markers, ScOPE16₁₆₀₀ and A5₄₂₀y, were mapped at 9.6 and 23.0 cM from the PMR locus, respectively. The novel markers identified in this study are currently being transferred to a new F2 mapping population derived from a cross between the induced PMR mutant line F(er1mut2) and a more genetically distant susceptible line of Pisum sativum var. arvense.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-2336eISSN: 1573-5060DOI: 10.1007/s10681-009-0003-8Titel-ID: cdi_proquest_miscellaneous_21271335
- Format
- –
- Schlagworte
- Agronomy. Soil science and plant productions, Airborne microorganisms, alleles, amplified fragment length polymorphism, Analysis, Arvenses, Biological and medical sciences, Biomarkers, Biomedical and Life Sciences, Biotechnology, Deoxyribonucleic acid, disease resistance, DNA, Erysiphe pisi, Ethylenediaminetetraacetic acid, ethylnitrosourea, Fundamental and applied biological sciences. Psychology, Gene mutations, Genetic aspects, Genetic markers, genetic resistance, Genetics and breeding of economic plants, host-pathogen relationships, induced resistance, isogenic lines, Legumes, Life Sciences, linkage groups, molecular sequence data, Mutagenesis, mutants, Mutation, nucleotide sequences, pathogenicity, Peas, Pest resistance, Pisum sativum, Pisum sativum var. arvense, plant breeding, Plant breeding: fundamental aspects and methodology, Plant diseases, Plant Genetics and Genomics, plant pathogenic fungi, Plant pathogens, Plant Pathology, Plant Physiology, Plant Sciences, powdery mildew, random amplified polymorphic DNA technique, Varietal selection. Specialized plant breeding, plant breeding aims