Details

Autor(en) / Beteiligte

• Aziz, Najib
• Butch, Anthony W.
• Ryner, Timothy C.
• Martinez-Maza, Otoniel
• Detels, Roger

Titel

The influence of EDTA Vacutainer blood collection tube on the level of blood interleukin-1 receptor antagonist

Ist Teil von

• Journal of immunological methods, 2019-01, Vol.464, p.114-118

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• The use of anticoagulants may influence the composition of blood cells and interfere with plasma levels of IL-1ra when unprocessed EDTA blood samples are stored for long periods of time. Blood was drawn into EDTA and heparinized blood collection tubes from 11 HIV-1 negative men participating in the Multicenter AIDS Cohort Study (MACS) and 4 healthy volunteers. The blood was processed according to the experiments detailed in the method and after incubation; supernatants were collected and stored at -70 °C until batch testing using IL-1ra ELISA. There was no difference between the levels of IL-1ra in EDTA blood collected into plastic and glass tubes (p = .911). There were significant increases from baseline levels of IL-1ra (p ≤ .05) after 24 h incubation for diluted whole blood and PBMC supernatants but not for granulocytes supernatants. EDTA as an anticoagulant influences the blood concentrations of IL-1ra in unprocessed blood. Thus, EDTA blood is not a suitable specimen for measurement of IL-1ra. Other types of anticoagulated blood should be processed within one hour of draw whenever measuring plasma levels of IL-1ra. •Time delay between blood draw and centrifugation of EDTA blood tube dramatically influence the measurement of IL-1ra.•The result of this study indicates that EDTA plasma will not be a suitable specimen for reliable measurement of IL-1ra.