Details

Autor(en) / Beteiligte

• Villabona-Monsalve, Juan P
• Varnavski, Oleg
• Palfey, Bruce A
• Goodson, Theodore

Titel

Two-Photon Excitation of Flavins and Flavoproteins with Classical and Quantum Light

Ist Teil von

• Journal of the American Chemical Society, 2018-11, Vol.140 (44), p.14562-14566

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2018

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• In this contribution, the entangled two-photon absorption (ETPA) process on naturally occurring flavoproteins was studied. Low temperature responsive protein (LOT6P) and b-type dihydroorotate dehydrogenase (DHOD B), which possess flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) chromophores embedded in the protein environment, were investigated. The ETPA cross-section was measured, and we found that it increases when going from an aqueous solution of the free flavin chromophore to the chromophore embedded in the protein. This enhancement is particularly evident when entangled photons are used as excitation light compared to classical light. Our results prove the potential of ETPA as a sensing technique for fluorescent proteins even for those whose classical TPA cross-section is small compared to well-known fluorescent proteins.