American journal of respiratory and critical care medicine, 2019-03, Vol.199 (5), p.592-602
2019
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- Autor(en) / Beteiligte
- Titel
- Increased IgA Expression in Lung Lymphoid Follicles in Severe Chronic Obstructive Pulmonary Disease
- Ist Teil von
- American journal of respiratory and critical care medicine, 2019-03, Vol.199 (5), p.592-602
- Ort / Verlag
- United States: American Thoracic Society
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Accumulation of B cells and lymphoid follicles (LFs) has been described in chronic obstructive pulmonary disease (COPD) airways, but the functional status of lung B cells remains poorly known. To characterize LFs for expression of IgA, the main mucosal antibody. The presence of B cells and LFs, including intrafollicular IgA expression, were determined in the lung from patients with COPD (n = 37) versus control subjects (n = 34) by immunohistochemistry. We also evaluated follicular IgA responses in the lungs from mice infected with Pseudomonas aeruginosa (PAO1) (n = 10 per group) and in smoking mice. Whereas in smokers B-cell numbers slightly increased, robust increases in B-cell and LF numbers (mainly in distal airways) were only observed in severe COPD. Most follicular B cells were IgM (70-80%), but IgA (and not IgG ) B-cell numbers were increased in LFs from severe COPD compared with control subjects (twofold, 44.7% vs. 25.2%), and this was significant in distal but not proximal airways. Follicular IgA response was also observed in PAO1-infected mouse lungs, but not after smoke exposure. Moreover, follicular IgA expression associated with expression of IL-21, which was very potent to activate immunoglobulin production in vitro. This study shows that IgA production occurs in peribronchiolar LFs from severe COPD, where IL-21-producing T cells are present, and presumably represents a feature of exacerbated mucosal adaptive immune responses against microbial and/or self-antigens.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1073-449XeISSN: 1535-4970DOI: 10.1164/rccm.201802-0352OCTitel-ID: cdi_proquest_miscellaneous_2123720708
- Format
- –
- Schlagworte
- Acute Disease, Animals, B-Lymphocytes - immunology, B-Lymphocytes - metabolism, B-Lymphocytes - pathology, Case-Control Studies, Chronic obstructive pulmonary disease, Cigarettes, Cytokines, Dendritic cells, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Humans, Immunoglobulin A - metabolism, Immunoglobulins, Infections, Interleukin-6 - metabolism, Interleukins - metabolism, Ligands, Lung - immunology, Lung - metabolism, Lung - pathology, Lung cancer, Lung diseases, Lymphocytes, Male, Mice, Inbred C57BL, Middle Aged, Pulmonary Disease, Chronic Obstructive - immunology, Pulmonary Disease, Chronic Obstructive - metabolism, Pulmonary Disease, Chronic Obstructive - pathology, Real-Time Polymerase Chain Reaction, Smoking, Smoking - adverse effects, Smoking - metabolism, Smoking - pathology, Surgery, Tertiary Lymphoid Structures - immunology, Tertiary Lymphoid Structures - metabolism, Tertiary Lymphoid Structures - pathology, Tobacco smoke