Details

Autor(en) / Beteiligte

• Cizkova, Jana
• Sinkorova, Zuzana
• Strnadova, Kristyna
• Cervinkova, Monika
• Horak, Vratislav
• Sinkora, Jiri
• Stepanova, Katerina
• Sinkora, Marek

Titel

The role of αβ T-cells in spontaneous regression of melanoma tumors in swine

Ist Teil von

• Developmental and comparative immunology, 2019-03, Vol.92, p.60-68

Ort / Verlag

Oxford: Elsevier Ltd

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Using a porcine model, we describe Melanoma-Associated CD4+CD8hi T-lymphocytes (MATL) in peripheral blood that increase during melanoma regression. These MATL possess the CD4+CD8hi phenotype and they have their direct counterparts in Tumor Infiltrating Lymphocytes (TIL) isolated from melanoma loci. Both MATL and CD4+CD8hi TIL have a similar expression of selected markers indicating that they represent effector/memory αβ T-cell subset. Moreover, although TIL also contain CD4—CD8+ T-cells, only CD4+CD8hi TIL expand during melanoma regression. Importantly, TIL isolated from different pigs and different melanoma loci among the same pig have similar composition of CD4/CD8 subsets, indicating that the composition of the MATL and TIL compartment is identical. Analysis of sorted cells from regressing pigs revealed a unique MATL subpopulation with mono-specific T-cell receptor that was further analyzed by sequencing. These results indicate that pigs regressing melanomas possess a characteristic population of recirculating T-cells playing a role in tumor control and regression. •Peripheral CD4+CD8hi αβ T cells (MATL) are involved in melanoma regression.•These MATL have their direct counterparts in Tumor Infiltrating Lymphocytes (TIL).•Both MATL and CD4+CD8hi TIL have similar expression of selected markers.•CD4/CD8 subsets isolated from different pigs and different melanoma loci are similar.•MATL possess mono-specific T-cell receptor that may be responsible for regression.