Details

Autor(en) / Beteiligte

• de León-Door, Adrián Ponce
• Romo-Chacón, Alejandro
• Rios-Velasco, Claudio
• Zamudio-Flores, Paul Baruk
• Ornelas-Paz, José de Jesús
• Acosta-Muñiz, Carlos H.

Titel

Prevalence, typing and phylogenetic analysis of Melissococcus plutonius strains from bee colonies of the State of Chihuahua, Mexico

Ist Teil von

• Journal of invertebrate pathology, 2018-11, Vol.159, p.71-77

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •Typical and atypical strains of Melissococcus plutonius were detected in beehives from Mexico.•Visual symptoms correlate with molecular detection of M. plutonius.•Three new STs of M. plutonius were identified.•First report in Mexico about the molecular characterization of M. plutonius. European foulbrood (EFB) caused by Melissococcus plutonius is an important bee brood disease but, in Mexico, information about this bacterium is limited. We evaluated the prevalence of typical and atypical strains in beehives of seven apicultural regions of the state of Chihuahua, Mexico. We performed MLST and phylogenetic analysis to characterize the isolates. Prevalence was highest 59%, in the region of Chihuahua, and lowest, 14%, in the regions of Cuauhtémoc and Nuevo Casas Grandes. Typical and atypical strains were identified in hives from all regions; however, in the regions of Parral, Cuauhtémoc and Aldama, the atypical strains were only detected in combination with typical strains. We obtained 81 isolates of M. plutonius and identified seven sequence types, of which three were new types. Additionally, we observed a relation between sequence type and the region where the strain was isolated. Phylogenetic analysis and multilocus sequence typing using goeBURST analysis showed that 97.5% of the isolates correspond to the Clonal Complex (CC) 12 and 2.5% to the CC3. Our work is the first molecular characterization of M. plutonius in Mexico and contributes to global information about the epidemiology of this pathogen.