Details

Autor(en) / Beteiligte

• Li, Jing
• Wang, Jun-Tao
• Hu, Hang-Wei
• Cai, Zhang-Jie
• Lei, Yin-Ru
• Li, Wei
• Zhang, Man-Yin
• Li, Zong-Ming
• Zhu, Yi-Nuo
• Cui, Li-Juan

Titel

Changes of the denitrifying communities in a multi-stage free water surface constructed wetland

Ist Teil von

• The Science of the total environment, 2019-02, Vol.650 (Pt 1), p.1419-1425

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Microorganisms play crucial roles in the nitrogen removal processes of wetlands. However, the key functional genes and microbes related to the nitrogen removal remain largely unknown in the free water surface constructed wetland (FWS CW). Here we studied the abundances of denitrifiers by targeting the key functional genes (nirS, nirK and nosZ) and investigated the community compositions of denitrifiers and their correlations with the abiotic variables in a FWS CW. The increase of nosZ/(nirS + nirK) and nirS/nirK ratios in the outlet indicated a shift of denitrifiers' communities which tended to release less nitrous oxide at the genetic potential level. The denitrifiers dominated the bacterial community which also remarkably changed from the inlet to the outlet. PICRUSt analysis revealed that the denitrifiers contributed to 39.1% of the nitrogen metabolism, 38.9% of the amino acid metabolism and 25.6% of the amino acid related enzymes. Four bacterial genera including Hydrogenophaga, Hylemonella, Aquabacterium and Cellvibrio were detected as the putative keystone denitrifiers. The abundance (nirS, nirK and nosZ) and the relative abundance of putative keystone denitrifiers were significantly correlated with total organic carbon, oxidation-reduction potential and C/N ratio, which could be regarded as the determinants for the denitrification process in the free water. [Display omitted] •Denitrifiers were investigated in a multi-stage free water surface constructed wetland.•The abundance of nosZ-type denitrifiers increased in the outlet.•The denitrifiers' community composition remarkably changed from the inlet to outlet.•Four putative keystone denitrifers were predictors of nitrogen related functions.