Brain research, 2009-12, Vol.1301, p.152-162
2009
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- Autor(en) / Beteiligte
- Titel
- Impaired blood–brain and blood–spinal cord barriers in mutant SOD1-linked ALS rat
- Ist Teil von
- Brain research, 2009-12, Vol.1301, p.152-162
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Blood–brain barrier (BBB) and blood–spinal cord barrier (BSCB) impairment is an additional accident occurring during the amyotrophic lateral sclerosis (ALS) progression. In this work, we aimed to decipher if BBB/BSCB leakage appeared before critical detrimental events and could serve as a marker preceding clinical symptoms. Three different BBB leakage markers: Evans blue, IgG and hemosiderin, were used to look at the SOD1-linked ALS rat model at presymptomatic and symptomatic stages. Although IgG and hemosiderin could be detected at presymptomatic stage, Evans blue extravasation which fits best with BBB/BSCB impairment could only be seen at symptomatic stages. BBB/BSCB impairment was further substantiate by showing at symptomatic stages decreased mRNA expression of ZO-1 and occludin as well as agrin, a basal membrane constituent. Electron microscopic data substantiate a toxic environment around endothelial cell and peri-vascular swollen astrocyte end-feet showing oedema-linked BBB opening.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-8993eISSN: 1872-6240DOI: 10.1016/j.brainres.2009.09.018Titel-ID: cdi_proquest_miscellaneous_21171663
- Format
- –
- Schlagworte
- Agrin - genetics, Agrin - metabolism, ALS, Amyotrophic Lateral Sclerosis - genetics, Amyotrophic Lateral Sclerosis - metabolism, Amyotrophic Lateral Sclerosis - pathology, Animals, Astrocytes - metabolism, Astrocytes - pathology, Biological and medical sciences, Biomarkers, Blood-Brain Barrier - metabolism, Blood-Brain Barrier - pathology, Blood–brain barrier, Blood–spinal cord barrier, Brain Stem - metabolism, Brain Stem - pathology, Cell Shape, Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges, Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases, Disease Models, Animal, Endothelial Cells - cytology, Enzyme-Linked Immunosorbent Assay, Evans Blue, Fundamental and applied biological sciences. Psychology, Hemosiderin - metabolism, Immunoglobulins - metabolism, Immunohistochemistry, Medical sciences, Membrane Proteins - genetics, Membrane Proteins - metabolism, Microscopy, Electron, Mutant SOD1, Neurology, Occludin, Phosphoproteins - genetics, Phosphoproteins - metabolism, Rat, Rats, Rats, Transgenic, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - genetics, RNA, Messenger - metabolism, Spinal Cord - metabolism, Spinal Cord - pathology, Staining and Labeling, Superoxide Dismutase - genetics, Vertebrates: nervous system and sense organs, Zonula Occludens-1 Protein