Nature biotechnology, 2018-12, Vol.36 (11), p.1059-1061
2018
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Details
- Autor(en) / Beteiligte
- Titel
- Comprehensive identification of peptides in tandem mass spectra using an efficient open search engine
- Ist Teil von
- Nature biotechnology, 2018-12, Vol.36 (11), p.1059-1061
- Ort / Verlag
- New York: Nature Publishing Group US
- Erscheinungsjahr
- 2018
- Beschreibungen/Notizen
- Peptide identification in proteomics data is improved using an efficient open search engine. We present a sequence-tag-based search engine, Open-pFind, to identify peptides in an ultra-large search space that includes coeluting peptides, unexpected modifications and digestions. Our method detects peptides with higher precision and speed than seven other search engines. Open-pFind identified 70–85% of the tandem mass spectra in four large-scale datasets and 14,064 proteins, each supported by at least two protein-unique peptides, in a human proteome dataset.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1087-0156eISSN: 1546-1696DOI: 10.1038/nbt.4236Titel-ID: cdi_proquest_miscellaneous_2117153915
- Format
- –
- Schlagworte
- 631/114/2784, 631/114/794, 631/1647/2067, 631/61/475, 82/58, Agriculture, Bioinformatics, Biomedical Engineering/Biotechnology, Biomedicine, Biotechnology, brief-communication, Life Sciences, Mass spectra, Mass spectrometry, Mass spectroscopy, Online information services, Peptides, Proteins, Proteomes, Proteomics, Search engines