Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
The p38‐activated ER stress‐ATF6α axis mediates cellular senescence
Ist Teil von
The FASEB journal, 2019-02, Vol.33 (2), p.2422-2434
Ort / Verlag
United States: Federation of American Societies for Experimental Biology
Erscheinungsjahr
2019
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
ABSTRACT
The importance of proteostasis in preventing cellular senescence has been well recognized. However, the exact mechanism by which the loss of proteostasis or endoplasmic reticulum (ER) stress induces cellular senescence remains unclear. We report that ER stress mediates cellular senescence through the activating transcription factor (ATF)6α branch of the unfolded protein response (UPR). Cellular senescence was induced by the abrogation of neighbor of breast cancer (BRCA)1 gene (NBR1). NBR1 abrogation‐induced senescence was p53 dependent and observed in both transformed and nontransformed human cell lines: MCF‐7, Caki‐1, and MRC‐5. NBR1 bound to p38 MAPK, preferentially to an active form, and upon NBR1 abrogation, the activity of p38 increased. NADPH oxidase was activated in turn by p38, and the resulting oxidative stress triggered ER stress. It was found that ER stress mediated cellular senescence through the UPR sensor ATF6α. Knockdown of ATF6α prevented senescence, whereas ATF6α overexpression triggered it. The transcriptional activity of ATF6α was important. The ER stress‐ATF6α axis also mediated cellular senescence induced by H‐RasV12 overexpression and UV irradiation, suggesting a common role of this axis in senescence induction. In summary, we presented an evidence for the novel role of the ER stress‐ATF6α axis in cellular senescence.—Kim, H. S., Kim, Y., Lim, M. J., Park, Y.‐G., Park, S. I., Sohn, J. The p38‐activated ER stress‐ATF6α axis mediates cellular senescence. FASEB J. 33, 2422–2434 (2019). www.fasebj.org